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One step Ovulation Test Instructions

TEST DESCRIPTION

One Step LH Urine Ovulation Test is fast and easy-to-use. It is a qualitative test that predicts LH (Luteinizing Hormone) surge, and in turn, when you are likely to ovulate and conceive. Females who are trying to become pregnant may use this information to improve their chances of conception. Luteinizing Hormone is a hormone released by the pituitary gland, a small gland at the base of the brain. The hormone stimulates the ovaries to produce and release eggs each month during the menstrual cycle. The level of LH in the blood and urine is highest before ovulation. This increase in hormone level is sometimes called a "surge". If fertility drugs are given to stimulate ovulation, an LH test can help determine the best time for sexual intercourse. The LH test may also be used to determine when eggs are mature enough to be surgically removed from the ovary as part of the in vitro fertilization process. LH tests may also aid in the diagnoses of polycystic ovary disease, premature ovarian failure, and menopause. The tests detect LH in urine in a qualitative format with sensitivity of 25mIU/mL (International Standard Value) and specificity is 99.8%. Tests have an expiry date of 2 years and can be stored in a dry environment ranging from 2 to 30 degrees Celsius. Results are available in just minutes. 

WHEN TO BEGIN TESTING

First, you must determine the length of your menstrual cycle. This is the number of days from the first day of your menstrual bleeding to the day before your next bleeding begins again. Please refer to the chart  to determine when you should start testing. If your cycle is shorter than 21 days or longer than 38 days, consult your doctor. If you do not know your cycle length, you may begin the test 11 days after your first period since the average cycle length is 28 days. Perform 1 test each day over a 5 day period or until the LH surge 
has been detected.    

URINE COLLECTION

Do not use first morning urine samples as LH is synthesized in your body early in the morning. It will not show up in your urine until later in the day.   The best time to collect your urine is between 10am - 8pm.  Collect urine at about the same time each day.  Reduce liquid intake about 2 hours before collecting your urine as a diluted urine sample can prevent the test from detecting LH surge.Please refer to the table below for information on when to start using ovulation tests:

TEST PROCEDURE: 

STEP 1
Collect urine in a clean container (such as plastic cup). The best way to collect sample is by placing a cup in the middle of urination process.                                              

STEP 2
Immerse the strip into the urine with the arrow end pointing towards the urine. Do not immerse past the "Mark" line. Take the strip out after 5 seconds and lay the strip flat on a clean, dry, non-absorbent surface (e.g., mouth of the urine container).                                                                                                       

STEP 3
Wait for colored bands to appear. Depending on the concentration of LH in the test specimen, positive results may be observed in as short as 40 seconds. However, to confirm negative results, the complete reaction time (10 minutes) is required. Do not read results after 30 minutes.

INTERPRETATION OF RESULTS

No LH Surge:
Only one color band appears on the control region or the test band is present but lighter in color intensity than the control band. There is no LH surge even if two lines present - as long as test line is fainter than the control line the result is negative.                                                                                       

LH Surge:
If two color bands are visible and the test band is equal to or darker than the control band, one will probably ovulate in the next 24-48 hours. If one wants to be pregnant, the best time to have intercourse is after 24 but before 48 hours.                                                                                                           

Invalid: Uncertain Results: No visible bands in the control and test regions. Make sure to follow the above specified instructions for optimum results.

Your cycle length----Start to test on

21 days-----------------Day 6
22 days-----------------Day 6
23 days-----------------Day 7
24 days-----------------Day 7
25 days-----------------Day 8
26 days-----------------Day 9
 
27 days----------------Day 10
 
28 days----------------Day 11
 
29 days----------------Day 12
 
30 days----------------Day 13
 
31 days----------------Day 14
 
32 days----------------Day 15
 
33 days----------------Day 16
 
34 days----------------Day 17
 
35 days----------------Day 18
 
36 days----------------Day 19

 37 days----------------Day 20
 
38 days----------------Day 21
 

                                                                                               

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